Track 1: Supporting the 3Rs by Increasing Catheter Patency Duration in Rodents

Vascular access is a key element in many preclinical studies. In chronic protocols involving repeated administration of compounds, blood sampling, or both, maintaining a prolonged catheter patency duration is essential. Several factors influence the catheter functionality, the most critical being the accurate positioning of the catheter tip at an optimal anatomical site. The use of a closed system is another crucial determinant; closed systems incorporating transcutaneous buttons have been shown to significantly extend catheter patency performance. Catheter material and tip design also play important roles. Polyurethane is widely considered the material of choice, while a rounded tip minimizes endothelial damage and reduces the risk of thrombus formation. Strict adherence to aseptic technique and the use of sterile solutions are essential to prevent infection and biofilm formation, thereby promoting an unobstructed catheter flow. In addition, employing a positive pressure technique during catheter locking decreases the likelihood of intraluminal clot formation. Collectively, these factors must be carefully considered and consistently applied to achieve optimal catheter patency duration.

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